Search results for "immuno MALDI-MS"

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Immuno-MALDI-MS in Human Plasma and on-Chip Biomarker Characterizations at the Femtomole Level

2012

Immuno-SPR-MS is the combination of immuno-sensors in biochip format with mass spectrometry. This association of instrumentation allows the detection and the quantification of proteins of interest by SPR and their molecular characterization by additional MS analysis. However, two major bottlenecks must be overcome for a wide diffusion of the SPR-MS analytical platform: (i) To warrant all the potentialities of MS, an enzymatic digestion step must be developed taking into account the spot formats on the biochip and (ii) the biological relevancy of such an analytical solution requires that biosensing must be performed in complex media. In this study, we developed a procedure for the detection …

immuno MALDI-MSMaldi mseducationlcsh:Chemical technologyProteomicsMass spectrometry01 natural sciencesBiochemistryArticleAnalytical Chemistry[SPI.MAT]Engineering Sciences [physics]/MaterialsAutomation03 medical and health sciencesproteomicsLimit of DetectionLab-On-A-Chip DevicesHumanslcsh:TP1-1185Electrical and Electronic Engineering[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/MicroelectronicsBiochipInstrumentationmass spectrometry030304 developmental biologyDetection limit[SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph]0303 health sciencesChromatographyChemistry010401 analytical chemistryReproducibility of ResultsSurface Plasmon ResonanceAtomic and Molecular Physics and Optics0104 chemical sciencesBiomarker (cell)Human plasmaSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationSurface Plasmon Resonance; mass spectrometry; immuno MALDI-MS; biomarker; proteomicsbiomarkerBiosensorBiomarkers
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